Journal: Magma (New York, N.Y.)
Article Title: Detection of axonal degeneration in a mouse model of Huntington’s disease: comparison between diffusion tensor imaging and anomalous diffusion metrics
doi: 10.1007/s10334-019-00742-6
Figure Lengend Snippet: Multi-cellular changes in the corpus callosum of the Huntington’s disease mice reveals increased white matter complexity. a Diagram showing coronal sections and histological regions of interest (ROIs in light blue) centered in the corpus callosum (CC) between the cortex (CCX) and the striatum. b Coronal sections centered in the corpus callosum showing changes in neuronal architecture and axonal orientation by endogenous expression. Yellow fluorescent protein (YFP) can be observed in the R6/2 mice (YFP, R6/2). Note an increase of axonal tortuosity in the R6/2 mice. Nuclear counterstaining with DAPI (Blue). Astrocyte proliferation is labeled by glial fibrillary acid protein (GFAP) and can be observed in white matter (WM) in the HD mice. The amount of myelin basic protein (MBP)—a marker of oligodendrocyte function—is decreased in the HD mice. c Quantitative fluorescence analysis of white matter markers (YFP, GFAP, and MBP) in the corpus callosum of the R6/2 and WT mice (***p < 0.001) (n = 6 mice per group). AU Arbitrary Units. Scale bar = 10 μm
Article Snippet: Sections were incubated with TBS containing primary antibodies recognizing myelin basic protein (MBP; PhosphoSolution, Cat #1120-MBP 1:500 Aurora, CO, USA) or the astrocyte maker glial fibrillary acid protein (GFAP; NeuroMab Cat #75–240 1:50, Davis, CA, USA).
Techniques: Expressing, Labeling, Marker, Fluorescence